Flow cytometric analysis of Exosome component 1 expression in HepG2 cells using Exosome component 1 antibody (M020630, 1:1,000). Green, isotype control; red, Exosome component 1.

Immunocytochemical staining of HepG2 cells with Exosome component 1 antibody (M020630, 1:500) . Nuclei were stained blue with DAPI; Exosome component 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using exosome component 1 antibody (M020630). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with exosome component 1 antibody (M020630, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using exosome component 1 antibody (M020630). Exosome component 1 expression in wild-type (WT) and exosome component 1 (EXOSC1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with exosome component 1 antibody (M020630, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of HeLa cells using Exosome component 1 antibody (M020630, 1:1,000), Top panel: wild-type (WT); Bottom panal: Exosome component 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.