[KD Validated] Anti-GBE1 Mouse mAb [15E43S34]

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[KD Validated] Anti-GBE1 Mouse mAb [15E43S34]

Purified Recombinant Mouse Monoclonal Antibody

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货号	规格	价格
M020615
	20µL	¥738.00
	50µL	¥1380.00
	100µL	¥2280.00

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[KD Validated] Anti-GBE1 Mouse mAb [15E43S34]

Flow cytometric analysis of GBE1 expression in HepG2 cells using GBE1 antibody (M020615, 1:1,000). Green, isotype control; red, GBE1.

Western blotting analysis using GBE1 antibody (M020615). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GBE1 antibody (M020615, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using GBE1 antibody (M020615). GBE1 expression in wild-type (WT) and GBE1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with GBE1 antibody (M020615, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Product Name	[KD Validated] Anti-GBE1 Mouse mAb
Description	Purified Recombinant Mouse Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC
Dilution	WB 1:500~1:2,500; FC 1:100~1:1,000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone No.	15E43S34
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant protein of human GBE1
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	GBE1; 1,4-Alpha-Glucan Branching Enzyme 1; Glucan (1,4-Alpha-), Branching Enzyme 1; 1,4-Alpha-Glucan-Branching Enzyme; Glycogen Branching Enzyme; Brancher Enzyme; EC 2.4.1.18; Amylo-(1,4 To 1,6) Transglucosidase; Amylo-(1,4 To 1,6) Transglycosylase; Glycogen Storage Disease Type IV; Glycogen-Branching Enzyme; Andersen Disease; APBD; GSD4; GBE.
Molecular weight	Calculated MW: 80 kDa; Observed MW: 70 kDa
Uniprot ID	Q04446
Gene ID	2632
Storage	Shipped on wet ice. Store at -20℃. Stable for 12 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	[KD Validated] Anti-GBE1 Mouse mAb [15E43S34] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	The protein encoded by this gene is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease).

Background

The protein encoded by this gene is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease).

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M020615 [KD Validated] Anti-GBE1 Mouse mAb [15E43S34]

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