[KD Validated] Anti-SNX9 Mouse mAb [54F66D77]

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[KD Validated] Anti-SNX9 Mouse mAb [54F66D77]

Purified Recombinant Mouse Monoclonal Antibody

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货号	规格	价格
M020596
	20µL	¥738.00
	50µL	¥1380.00
	100µL	¥2280.00

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[KD Validated] Anti-SNX9 Mouse mAb [54F66D77]

Flow cytometric analysis of SNX9 expression in HeLa cells using SNX9 antibody (M020596, 1:2,000). Green, isotype control; red, SNX9.

Immunocytochemical staining of Hela cells with SNX9 antibody (M020596, 1:1,000). Nuclei were stained blue with DAPI; SNX9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using SNX9 antibody (M020596). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SNX9 antibody (M020596, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using SNX9 antibody (M020596). SNX9 expression in wild-type (WT) and SNX9 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SNX9 antibody (M020596, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of HeLa cells using SNX9 antibody (M020596, 1:1,000), Top panel: wild-type (WT); Bottom panal: SNX9 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SNX9 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Product Name	[KD Validated] Anti-SNX9 Mouse mAb
Description	Purified Recombinant Mouse Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IF (Cell)/ICC
Dilution	WB 1:500~1:2,500; FC 1:200~1:2,000; IF 1:100~1:1,000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone No.	54F66D77
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant protein of human SNX9
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	SNX9; Sorting Nexin 9; SH3PXD3A; SH3PX1; SDP1; SH3 And PX Domain-Containing Protein 3A; SH3 And PX Domain-Containing Protein 1; Sorting Nexin-9; Wiskott-Aldrich Syndrome Protein (WASP) Interactor Protein; Protein SDP1; WISP.
Molecular weight	Calculated MW: 67 kDa; Observed MW: 74 kDa
Uniprot ID	Q9Y5X1
Gene ID	51429
Storage	Shipped on wet ice. Store at -20℃. Stable for 12 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	[KD Validated] Anti-SNX9 Mouse mAb [54F66D77] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This gene encodes a member of the sorting nexin family. Members of this family contain a phosphoinositide binding domain, and are involved in intracellular trafficking. The encoded protein does not contain a coiled coil region, like some family members, but does contain a SRC homology domain near its N-terminus. The encoded protein is reported to have a variety of interaction partners, including of adaptor protein 2 , dynamin, tyrosine kinase non-receptor 2, Wiskott-Aldrich syndrome-like, and ARP3 actin-related protein 3. The encoded protein is implicated in several stages of intracellular trafficking, including endocytosis, macropinocytosis, and F-actin nucleation. [provided by RefSeq, Jul 2013]

Background

This gene encodes a member of the sorting nexin family. Members of this family contain a phosphoinositide binding domain, and are involved in intracellular trafficking. The encoded protein does not contain a coiled coil region, like some family members, but does contain a SRC homology domain near its N-terminus. The encoded protein is reported to have a variety of interaction partners, including of adaptor protein 2 , dynamin, tyrosine kinase non-receptor 2, Wiskott-Aldrich syndrome-like, and ARP3 actin-related protein 3. The encoded protein is implicated in several stages of intracellular trafficking, including endocytosis, macropinocytosis, and F-actin nucleation. [provided by RefSeq, Jul 2013]

Cellular Location	Cytoplasmic vesicle membrane.Peripheral membrane protein.Cytoplasmic side.Cell membrane.Peripheral membrane protein.Cytoplasmic side.Cytoplasmic vesicle.Clathrin-coated vesicle.Golgi apparatus.trans-Golgi network.Cell projection.Ruffle.Cytoplasm.Localized at sites of endocytosis at the cell membrane. Detected on newly formed macropinosomes. Transiently recruited to clathrin-coated pits at a late stage of clathrin-coated vesicle formation. Colocalizes with the actin cytoskeleton at the cell membrane.

Cellular Location

Cytoplasmic vesicle membrane.Peripheral membrane protein.Cytoplasmic side.Cell membrane.Peripheral membrane protein.Cytoplasmic side.Cytoplasmic vesicle.Clathrin-coated vesicle.Golgi apparatus.trans-Golgi network.Cell projection.Ruffle.Cytoplasm.Localized at sites of endocytosis at the cell membrane. Detected on newly formed macropinosomes. Transiently recruited to clathrin-coated pits at a late stage of clathrin-coated vesicle formation. Colocalizes with the actin cytoskeleton at the cell membrane.

Tissue Location	Widely expressed, with highest levels in heart and placenta, and lowest levels in thymus and peripheral blood leukocytes.

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M020596 [KD Validated] Anti-SNX9 Mouse mAb [54F66D77]

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