Western blotting analysis using UBE3A antibody (M020429). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBE3A antibody (M020429, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using UBE3A antibody (M020429). UBE3A expression in wild-type (WT) and UBE3A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with UBE3A antibody (M020429, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of HAP-1 cells with UBE3A antibody(M020429, 1:1,000) . Nuclei were stained blue with DAPI; UBE3A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Flow cytometric analysis of UBE3A expression in HAP-1 cells using UBE3A antibody (M020429, 1:2,000). Green, isotype control; red, UBE3A.