Flow cytometric analysis of ARHGEF1 expression in HepG2 cells using ARHGEF1 antibody (M020426, 1:2,000). Green, isotype control; red, ARHGEF1.

Immunocytochemical staining of HepG2 cells with ARHGEF1 antibody (M020426, 1:1,000). Nuclei were stained blue with DAPI; ARHGEF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using ARHGEF1 antibody (M020426). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ARHGEF1 antibody (M020426, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using ARHGEF1 antibody (M020426). ARHGEF1 expression in wild-type (WT) and ARHGEF1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ARHGEF1 antibody (M020426, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of ARHGEF1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ARHGEF1 antibody (M020426, 1:2,000) and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using ARHGEF1 antibody (M020426, 1:1,000), Top panel: wild-type (WT); Bottom panal: ARHGEF1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Immunohistochemistry was performed on paraffin-embedded human lymphoma using ARHGEF1 antibody (M020426, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.