Western blotting analysis using MRI1 antibody (M020367). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MRI1 antibody (M020367, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using MRI1 antibody (M020367). MRI1 expression in wild-type (WT) and MRI1 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MRI1 antibody (M020367, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of HAP-1 cells with MRI1 antibody(M020367, 1:1,000) . Nuclei were stained blue with DAPI; MRI1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Validation of MRI1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with MRI1 antibody (M020367, 1:2,000) and analyzed using BD flow cytometer.

Flow cytometric analysis of MRI1 expression in HAP-1 cells using MRI1 antibody (M020367, 1:2,000). Green, isotype control; red, MRI1.

Immunocytochemical staining of HeLa cells using MRI1 antibody (M020367, 1:1,000), Top panel: wild-type (WT); Bottom panal: MRI1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.