Validation of TANK knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with TANK antibody (M020347, 1:2,000) and analyzed using BD flow cytometer.

Flow cytometric analysis of TANK expression in HepG2 cells using TANK antibody (M020347, 1:2,000). Green, isotype control; red, TANK.

Western blotting analysis using TANK antibody (M020347). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TANK antibody (M020347, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using TANK antibody (M020347). TANK expression in wild-type (WT) and TANK shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with TANK antibody (M020347, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.