Western blotting analysis using NFKB inhibitor alpha antibody (M020076). NFKB inhibitor alpha expression in wild type (WT) and NFKB inhibitor alpha (NFKBIA) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NFKB inhibitor alpha antibody (M020076, 1:5,000) and HRP-conjugated goat mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using NFKB inhibitor alpha antibody (M020076). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NFKB inhibitor alpha antibody (M020076, 1:2,500) and HRP-conjugated goat mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of C2C12 cells with NFKB inhibitor alpha antibody (M020076, 1:1,000). Nuclei were stained blue with DAPI; was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.