Anti-BID Mouse mAb [91H54T38]

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Anti-BID Mouse mAb [91H54T38]

Purified Recombinant Mouse Monoclonal Antibody

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货号	规格	价格
M016269
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-BID Mouse mAb [91H54T38]

All lanes: M016269 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: Hel ( Human erythroLeukemia suspension cell) whole cell lysates

Lane 4: Ball-1 (Human B lymphocyte acute leukemia cell) whole cell lysates

Lane 5: K562(Human chronic myeloid leukemia cell) whole cell lysates

Lane 6: THP-1(Human monocytic leukemia cell) whole cell lysates

Lane 7: U87(Human malignant glioblastoma epithelial cell) whole cell lysates

Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP Conjugated (Cat. No. LF101) at 1:5,000 dilution

Lysates/proteins at 10 µg per lane.

Predicted band size: 22 kDa

Observed band size: 20 kDa

Developed using the ECL technique (Cat. No. SQ201).

Product Name	Anti-BID Mouse mAb
Description	Purified Recombinant Mouse Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, ELISA
Dilution	WB 1:1,000~1:2,000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone No.	91H54T38
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant protein corresponding to Human BID
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	BH3-interacting domain death agonist; p22 BID; BID.
Molecular weight	Calculated MW: 22 kDa; Observed MW: 20 kDa
Uniprot ID	P55957
Gene ID	637
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-BID Mouse mAb [91H54T38] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2, and thus regulate apoptosis. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Aug 2020]

Background

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2, and thus regulate apoptosis. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Aug 2020]

Cellular Location	Cytoplasm. Mitochondrion membrane. Mitochondrion outer membrane. Note=When uncleaved, it is predominantly cytoplasmic.

Tissue Location	[Isoform 2]: Expressed in spleen, pancreas and placenta (at protein level). [Isoform 3]: Expressed in lung, pancreas and spleen (at protein level). [Isoform 4]: Expressed in lung and pancreas (at protein level).

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M016269 Anti-BID Mouse mAb [91H54T38]

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