Western Blot - Anti-SCAP Mouse mAb [17D22K25]

All lanes: M014936 at 1:4,000 dilution

Lane 1: A549 (Human lung carinoma epithelial cell) whole cell lysates

Lane 2: Caco2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates

Lane 3: Jurkat (Human T lymphocytic leukemia cell) whole cell lysates

Lane 4: 293T (Human embryonic kidney cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP Conjugated (Cat. No. LF101) at 1:5,000 dilution

Predicted band size: 140 kDa

Observed band size: 140 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-SCAP Mouse mAb [17D22K25]

Sample: Paraformaldehyde-fixed, paraffin embedded human breast cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: M014936 at 1:200 dilution

Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-SCAP Mouse mAb [17D22K25]

Sample: Paraformaldehyde-fixed, paraffin embedded human gastric cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: M014936 at 1:200 dilution

Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.