Anti-TATA Box Binding Protein Mouse mAb [55K89S00]

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Anti-TATA Box Binding Protein Mouse mAb [55K89S00]

Purified Recombinant Mouse Monoclonal Antibody

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货号	规格	价格
M014830
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-TATA Box Binding Protein Mouse mAb [55K89S00]

All lanes: M014830 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: U2OS (Human osteosarcoma epithelial cell) whole cell lysates

Lane 5: Caco2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates

Lane 6: Jurkat (Human T lymphocytic leukemia cell) whole cell lysates

Lane 7: 293T (Human embryonic kidney cell) whole cell lysates

Lane 8: SCC-9 (Human tongue squamous carcinoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP Conjugated (Cat. No. LF101) at 1:5,000 dilution

Predicted band size: 38 kDa

Observed band size: 35,45 kDa

Developed using the ECL technique (Cat. No. SQ201).

Western Blot - Anti-TATA Box Binding Protein Mouse mAb [55K89S00]

All lanes: M014830 at 1:1,000 dilution

Lane 9: C2C12 (Mouse myoblasts epithelial cell) whole cell lysates

Lane10: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates

Lane11: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP Conjugated (Cat. No. LF101) at 1:5,000 dilution

Predicted band size: 38 kDa

Observed band size: 35,45 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-TATA Box Binding Protein Mouse mAb [55K89S00]

Sample: HeLa cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibody: M014830 at 1:100 dilution

Secondary antibody: Goat anti-Mouse (488) at 1:1,000 dilution (shown in green)

Nuclei were stained with DAPI (shown in blue).

Immunohistochemistry - Anti-TATA Box Binding Protein Mouse mAb [55K89S00]

Sample: Paraformaldehyde-fixed, paraffin embedded rat testis tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: M014830 at 1:300 dilution

Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

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说明书
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Product Name	Anti-TATA Box Binding Protein Mouse mAb
Description	Purified Recombinant Mouse Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), IF (Cell)/ICC, ELISA
Dilution	WB 1:1,000~1:2,000; IHC-P 1:100~1:300; IF 1:100~1:200
Reactivity	Human, Mouse (Cell), Rat
Host	Mouse
Clonality	Monoclonal
Clone No.	55K89S00
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant protein of human TATA Box Binding Protein
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	GTF2D, GTF2D1, TFIID, MGC117320, MGC126054, MGC126055, SCA 17, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TATA sequence-binding protein, TATA binding protein TBP, TATA-binding factor, TATA-box factor, TATA-box-binding protein, TBP, TBP_HUMAN, TF2D, TFIID, Transcription initiation factor TFIID TBP subunit.
Molecular weight	Calculated MW: 38 kDa; Observed MW: 35,45 kDa
Uniprot ID	P20226
Gene ID	6908
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-TATA Box Binding Protein Mouse mAb [55K89S00] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 25-42, and expansion of the number of repeats to 45-66 increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2016]

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 25-42, and expansion of the number of repeats to 45-66 increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2016]

Cellular Location	Nuclear

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M014830 Anti-TATA Box Binding Protein Mouse mAb [55K89S00]

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