Anti-CaMKII beta Mouse mAb [80R01S35]

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Anti-CaMKII beta Mouse mAb [80R01S35]

Purified Recombinant Mouse Monoclonal Antibody

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货号	规格	价格
M014819
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-CaMKII beta Mouse mAb [80R01S35]

All lanes: M014819 at 1:2,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: U2OS (Human osteosarcoma epithelial cell) whole cell lysates

Lane 5: Caco2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates

Lane 6: Jurkat (Human T lymphocytic leukemia cell) whole cell lysates

Lane 7: 293T (Human embryonic kidney cell) whole cell lysates

Lane 8: SCC-9 (Human tongue squamous carcinoma epithelial cell) whole cell lysates

Lane 9: C2C12 (Mouse myoblasts epithelial cell) whole cell lysates

Lane10: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates

Lane11: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP Conjugated (Cat. No. LF101) at 1:5,000 dilution

Predicted band size: 73 kDa

Observed band size: 60 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-CaMKII beta Mouse mAb [80R01S35]

Sample: HeLa cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibody: M014819 at 1:100 dilution

Secondary antibody: Goat anti-Mouse (488) at 1:1,000 dilution (shown in green)

Nuclei were stained with DAPI (shown in blue).

Immunohistochemistry - Anti-CaMKII beta Mouse mAb [80R01S35]

Sample: Paraformaldehyde-fixed, paraffin embedded human hepatoma tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: M014819 at 1:300 dilution

Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

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Product Name	Anti-CaMKII beta Mouse mAb
Description	Purified Recombinant Mouse Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IF (Cell)/ICC, WB, IHC-P/IF (Tissue-P), ELISA
Dilution	WB 1:1,000~1:2,000; IHC-P 1:100~1:300; IF 1:100~1:200
Reactivity	Human, Mouse (Cell), Rat
Host	Mouse
Clonality	Monoclonal
Clone No.	80R01S35
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant protein of human CaMKII beta
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	Calcium/calmodulin dependent protein kinase (CaM kinase) II beta, Calcium/calmodulin dependent protein kinase II beta, Calcium/calmodulin dependent protein kinase IIB, Calcium/calmodulin dependent protein kinase type II beta chain, calcium/calmodulin-dependent protein kinase II beta, Calcium/calmodulin-dependent protein kinase type II subunit beta, CAM 2, CaM kinase II beta chain, CaM kinase II beta subunit, CaM kinase II subunit beta, CaM-kinase II beta chain, CAM2, CAMK 2, CAMK 2B, CaMK II beta subunit, CaMK II subunit beta, CaMK-II subunit beta, CAMK2, CaMK2 beta, Camk2b, CAMKB, CaMKII beta subunit, CaMKIIB, KCC2B_HUMAN, MGC29528, Proline rich calmodulin dependent protein kinase, proline rich calmodulin-dependent protein kinase.
Molecular weight	Calculated MW: 73 kDa; Observed MW: 60 kDa
Uniprot ID	Q13554
Gene ID	816
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-CaMKII beta Mouse mAb [80R01S35] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008]

Background

The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008]

Tissue Location	Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain.

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M014819 Anti-CaMKII beta Mouse mAb [80R01S35]

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