Purified Recombinant Mouse Monoclonal Antibody
| 货号 | 规格 | 价格 |
|---|---|---|
| M014706 | ||
| 20µL | ¥588.00 | |
| 50µL | ¥1080.00 | |
| 100µL | ¥1780.00 |
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![Anti-CDK2 Mouse mAb [90B23K63]](https://files.epizyme.cn:5005/uploads/productimage/M014706-1.png)
| Product Name | Anti-CDK2 Mouse mAb |
|---|---|
| Description | Purified Recombinant Mouse Monoclonal Antibody |
Application
|
WB, ELISA |
| Dilution | WB 1:1,000~1:2,000 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 90B23K63 |
| Isotype | IgG |
| Label | Unconjugated |
| Immunogen | Recombinant protein of human Cytokeratin CDK2 |
| Format | Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3. |
| Synonyms | Cdc2 related protein kinase, cdc2-related protein kinase, CDC28, CDC2A, Cdk 2, CDK1, CDK2, CDK2_HUMAN, CDKN2, Cell devision kinase 2, Cell division protein kinase 2, Cyclin dependent kinase 2, cyclin dependent kinase 2-alpha, Cyclin-dependent kinase 2, kinase Cdc2, MPF, p33 protein kinase, p33(CDK2). |
|---|---|
| Molecular weight | Calculated MW: 34 kDa; Observed MW: 34 kDa |
| Uniprot ID | P24941 |
| Gene ID | 1017 |
| Storage | Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage. |
| Precautions | Anti-CDK2 Mouse mAb [90B23K63] is for research use only and not for use in diagnostic or therapeutic procedures. |
| Background | Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity). Phosphorylates CDK2AP2 (PubMed:<a href="http://www.uniprot.org/citations/12944431" target="_blank">12944431</a>). Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks (PubMed:<a href="http://www.uniprot.org/citations/29203878" target="_blank">29203878</a>). |
|---|
| Cellular Location | Cytoplasm, cytoskeleton, centrosome. Nucleus, Cajal body. Cytoplasm. Endosome. Note=Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3). |
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