Anti-Rac1 Mouse mAb [34N20L43]

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Anti-Rac1 Mouse mAb [34N20L43]

Purified Recombinant Mouse Monoclonal Antibody

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货号	规格	价格
M014680
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-Rac1 Mouse mAb [34N20L43]

All lanes: M014680 at 1:1,000 dilution

Lane 1: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates

Lane 2: C2C12 (Mouse myoblasts epithelial cell) whole cell lysates

Lane 3: Rat spleen whole tissue lysates

Lane 4: Mouse small intestine whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP Conjugated (Cat. No. LF101) at 1:5,000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-Rac1 Mouse mAb [34N20L43]

Sample: Paraformaldehyde-fixed, paraffin embedded human breast cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: M014680 at 1:200 dilution

Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Product Name	Anti-Rac1 Mouse mAb
Description	Purified Recombinant Mouse Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), ELISA
Dilution	WB 1:1,000~1:2,000; IHC-P 1:100~1:200; IF 1:100~1:200
Reactivity	Mouse (Cell), Human, Rat
Host	Mouse
Clonality	Monoclonal
Clone No.	34N20L43
Isotype	IgG
Label	Unconjugated
Immunogen	A synthesized peptide derived from human Rac1
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	Cell migration inducing gene 5 protein, Cell migration-inducing gene 5 protein, MGC111543, MIG5, Migration inducing gene 5, Migration inducing protein 5, p21 Rac1, p21-Rac1, Rac 1, RAC1, RAC1_HUMAN, Ras like protein TC25, Ras related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1), Ras-like protein TC25, Ras-related C3 botulinum toxin substrate 1, Rho family small GTP binding protein Rac1, TC 25, TC25.
Molecular weight	Calculated MW: 21 kDa; Observed MW: 21 kDa
Uniprot ID	P63000
Gene ID	5879
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Rac1 Mouse mAb [34N20L43] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles (PubMed:<a href="http://www.uniprot.org/citations/1643658" target="_blank">1643658</a>, PubMed:<a href="http://www.uniprot.org/citations/28886345" target="_blank">28886345</a>). Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity (PubMed:<a href="http://www.uniprot.org/citations/9121475" target="_blank">9121475</a>). In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts (PubMed:<a href="http://www.uniprot.org/citations/1643658" target="_blank">1643658</a>). In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In neurons, is involved in dendritic spine formation and synaptic plasticity (By similarity). In hippocampal neurons, involved in spine morphogenesis and synapse formation, through local activation at synapses by guanine nucleotide exchange factors (GEFs), such as ARHGEF6/ARHGEF7/PIX (PubMed:<a href="http://www.uniprot.org/citations/12695502" target="_blank">12695502</a>). In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3. In neurons, plays a crucial role in regulating GABA(A) receptor synaptic stability and hence GABAergic inhibitory synaptic transmission through its role in PAK1 activation and eventually F-actin stabilization (By similarity).

Background

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles (PubMed:<a href="http://www.uniprot.org/citations/1643658" target="_blank">1643658</a>, PubMed:<a href="http://www.uniprot.org/citations/28886345" target="_blank">28886345</a>). Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity (PubMed:<a href="http://www.uniprot.org/citations/9121475" target="_blank">9121475</a>). In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts (PubMed:<a href="http://www.uniprot.org/citations/1643658" target="_blank">1643658</a>). In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In neurons, is involved in dendritic spine formation and synaptic plasticity (By similarity). In hippocampal neurons, involved in spine morphogenesis and synapse formation, through local activation at synapses by guanine nucleotide exchange factors (GEFs), such as ARHGEF6/ARHGEF7/PIX (PubMed:<a href="http://www.uniprot.org/citations/12695502" target="_blank">12695502</a>). In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3. In neurons, plays a crucial role in regulating GABA(A) receptor synaptic stability and hence GABAergic inhibitory synaptic transmission through its role in PAK1 activation and eventually F-actin stabilization (By similarity).

Cellular Location	Cell membrane. Melanosome. Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Tissue Location	Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.

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M014680 Anti-Rac1 Mouse mAb [34N20L43]

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