Anti-ATG4D Mouse mAb [74G68S88]

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Anti-ATG4D Mouse mAb [74G68S88]

Purified Recombinant Mouse Monoclonal Antibody

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货号	规格	价格
M014546
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-ATG4D Mouse mAb [74G68S88]

All lanes: M014546 at 1:4,000 dilution

Lane 1: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates

Lane 2: C2C12 (Mouse myoblasts epithelial cell) whole cell lysates

Lane 3: U2OS (Human osteosarcoma epithelial cell) whole cell lysates

Lane 4: Rat heart whole tissue lysates

Lane 5: Mouse small intestine whole tissue lysates

Lane 6: Mouse muscle whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP Conjugated (Cat. No. LF101) at 1:5,000 dilution

Predicted band size: 53 kDa

Observed band size: 53 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-ATG4D Mouse mAb [74G68S88]

Sample: HeLa cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibody: M014546 at 1:100 dilution

Secondary antibody: Goat anti-Mouse (488) at 1:1,000 dilution (shown in green)

Nuclei were stained with DAPI (shown in blue).

Product Name	Anti-ATG4D Mouse mAb
Description	Purified Recombinant Mouse Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF (Cell)/ICC, ELISA
Dilution	WB 1:1,000~1:4,000; IF 1:100~1:200
Reactivity	Human, Mouse (Cell), Rat
Host	Mouse
Clonality	Monoclonal
Clone No.	74G68S88
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant protein of human ATG4D
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	9830134P12Rik, APG4 autophagy 4 homolog D, APG4 D, APG4, S. cerevisiae, homolog of, D, APG4D, ATG4 autophagy related 4 homolog D (S. cerevisiae), Atg4d, ATG4D_HUMAN, Atg4dl, AUT like 4 cysteine endopeptidase, AUT-like 4 cysteine endopeptidase, AUTL4, Autophagin 4, Autophagin-4, autophagy 4, S. cerevisiae, homolog of, D, autophagy related 4D, cysteine peptidase, Autophagy related cysteine endopeptidase 4, Autophagy related protein 4 homolog D, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D, Cysteine protease ATG4D, Cysteine protease involved in autophagy, MGC31226.
Molecular weight	Calculated MW: 53 kDa; Observed MW: 53 kDa
Uniprot ID	Q86TL0
Gene ID	84971
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-ATG4D Mouse mAb [74G68S88] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013].

Background

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013].

Cellular Location	Cytoplasm.

Tissue Location	Mainly expressed in skeletal muscle and, to a lower extent, in testis.

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M014546 Anti-ATG4D Mouse mAb [74G68S88]

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