Anti-Hsp70 Mouse mAb [72Q64C01]

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Anti-Hsp70 Mouse mAb [72Q64C01]

Purified Recombinant Mouse Monoclonal Antibody

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货号	规格	价格
M014545
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-HSPA1A Mouse mAb [72Q64C01]

All lanes: M014545 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates

Lane 3: C2C12 (Mouse myoblasts epithelial cell) whole cell lysates

Lane 4: Rat spleen whole tissue lysates

Lane 5: Mouse small intestine whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP Conjugated (Cat. No. LF101) at 1:5,000 dilution

Predicted band size: 70 kDa

Observed band size: 70 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-HSPA1A Mouse mAb [72Q64C01]

Sample: HeLa cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibody: M014545 at 1:100 dilution

Secondary antibody: Goat anti-Mouse (488) at 1:1,000 dilution (shown in green)

Nuclei were stained with DAPI (shown in blue).

Product Name	Anti-Hsp70 Mouse mAb
Description	Purified Recombinant Mouse Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, ELISA, IF (Cell)/ICC
Dilution	WB 1:1,000~1:2,000; IF 1:100~1:200
Reactivity	Human, Mouse (Cell), Rat
Host	Mouse
Clonality	Monoclonal
Clone No.	72Q64C01
Isotype	IgG
Label	Unconjugated
Immunogen	A synthesized peptide derived from human HSPA1A
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	DnaK type molecular chaperone HSP70 1, Epididymis secretory protein Li 103, FLJ54303, FLJ54370, FLJ54392, FLJ54408, FLJ75127, Heat shock 70 kDa protein 1, Heat shock 70 kDa protein 1/2, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1A/1B, Heat shock 70kDa protein 1B, Heat shock induced protein, HEL S 103, HSP70 1, HSP70 1A, HSP70 1B, HSP70 2, HSP70-1, HSP70-1/HSP70-2, HSP70-1A, HSP70-2, HSP70.1, HSP70.1/HSP70.2, HSP71_HUMAN, HSP72, HSPA1, HSPA1A, HSPA1B.
Molecular weight	Calculated MW: 70 kDa; Observed MW: 70 kDa
Uniprot ID	P0DMV8
Gene ID	3303 , 3304
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Hsp70 Mouse mAb [72Q64C01] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008].

Background

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008].

Cellular Location	Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

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M014545 Anti-Hsp70 Mouse mAb [72Q64C01]

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