Anti-DRP1 Mouse mAb [35K09L62]

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Anti-DRP1 Mouse mAb [35K09L62]

Purified Recombinant Mouse Monoclonal Antibody

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货号	规格	价格
M013321
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Immunofluorescence - Anti-DRP1 Mouse mAb [35K09L62]

Sample: HeLa cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibody: M013321 at 1:100 dilution and and α-tubulin Rabbit Monoclonal Antibody (Cat. No. LF213) at 1:100 dilution

Secondary antibody: Goat anti-Mouse (488) at 1:1,000 dilution (shown in green) and Goat anti-Rabbit (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Western Blot - Anti-DRP1 Mouse mAb [35K09L62]

All lanes: M013321 at 1:1,000 dilution

Lane 1: Mouse brain whole tissue lysates

Lane 2: Rat brain whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Mouse IgG(H+L), HRP Conjugated (Cat. No. LF101) at 1:5,000 dilution

Predicted band size: 82 kDa

Observed band size: 82 kDa

Developed using the ECL technique (Cat. No. SQ201).

Product Name	Anti-DRP1 Mouse mAb
Description	Purified Recombinant Mouse Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF (Cell)/ICC, ELISA
Dilution	WB 1:1,000~1:2,000; IF 1:100~1:200
Reactivity	Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Clone No.	35K09L62
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant protein of human DRP1
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	Dynamin family member proline-rich carboxyl-terminal domain less; DVLP; Dynamin-like protein IV; Dynamin-related protein 1; Dynamin-like protein; DLP1; Dymple; Dynamin-1-like protein; HdynIV; Dnm1p/Vps1p-like protein; DRP1; DNM1L; Dynamin-like protein 4.
Molecular weight	Calculated MW: 82 kDa; Observed MW: 82 kDa
Uniprot ID	O00429
Gene ID	10059
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-DRP1 Mouse mAb [35K09L62] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]

Background

This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]

Cellular Location	Cytoplasm Cytosol Golgi apparatus Endomembrane system Peripheral membrane protein Mitochondrion outer membrane Peripheral membrane protein Peroxisome Membrane Clathrin-coated pit Cytoplasmic vesicle Secretory vesicle Synaptic vesicle membrane Mainly cytosolic. Recruited by RALA and RALBP1 to mitochondrion during mitosis (PubMed:21822277). Translocated to the mitochondrial membrane through O-GlcNAcylation and interaction with FIS1. Colocalized with MARCHF5 at mitochondrial membrane (PubMed:17606867). Localizes to mitochondria at sites of division (PubMed:15208300). Localizes to mitochondria following necrosis induction. Recruited to the mitochondrial outer membrane by interaction with MIEF1. Mitochondrial recruitment is inhibited by C11orf65/MFI (By similarity). Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear (PubMed:9422767, PubMed:9570752). In some cell types, localizes to the Golgi complex (By similarity). Binds to phospholipid membranes (By similarity).

Cellular Location

Cytoplasm Cytosol Golgi apparatus Endomembrane system Peripheral membrane protein Mitochondrion outer membrane Peripheral membrane protein Peroxisome Membrane Clathrin-coated pit Cytoplasmic vesicle Secretory vesicle Synaptic vesicle membrane Mainly cytosolic. Recruited by RALA and RALBP1 to mitochondrion during mitosis (PubMed:21822277). Translocated to the mitochondrial membrane through O-GlcNAcylation and interaction with FIS1. Colocalized with MARCHF5 at mitochondrial membrane (PubMed:17606867). Localizes to mitochondria at sites of division (PubMed:15208300). Localizes to mitochondria following necrosis induction. Recruited to the mitochondrial outer membrane by interaction with MIEF1. Mitochondrial recruitment is inhibited by C11orf65/MFI (By similarity). Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear (PubMed:9422767, PubMed:9570752). In some cell types, localizes to the Golgi complex (By similarity). Binds to phospholipid membranes (By similarity).

Tissue Location	Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons.

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M013321 Anti-DRP1 Mouse mAb [35K09L62]

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