Western Blot - Anti-Mannose Phosphate Isomerase Mouse mAb [15K89K18]
All lanes: M011823 at 1:1,000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates
Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates
Lane 4: MCF-7 (human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 5: 293T (Human embryonic kidney cell) whole cell lysates
Lane 6: U87 (Human malignant glioblastoma epithelial cells) whole cell lysates
Lane 7: C2C12 (Mouse myoblasts epithelial cell) whole cell lysates
Lane 8: Mouse brain whole tissue lysates
Lane 9: Mouse heart whole tissue lysates
Lane 10: Mouse liver whole tissue lysates
Lane 11: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates
Lane 12: Rat brain whole tissue lysates
Lane 13: Rat heart whole tissue lysates
Lane 14: Rat liver whole tissue lysates
Lysates/proteins at 10 µg per lane.
Secondary antibody: Goat Anti-Mouse IgG(H+L), HRP Conjugated (Cat. No. LF101) at 1:5,000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Developed using the ECL technique (Cat. No. SQ201).