Western Blot - Anti-Hsp90 Mouse mAb [91M01L08]

All lanes: M011208 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: MCF-7 (human breast adenocarcinoma epithelial cell) whole cell lysates

Lane 5: 293T (Human embryonic kidney cell) whole cell lysates

Lane 6: U87 (Human malignant glioblastoma epithelial cells) whole cell lysates

Lane 7: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates

Lane 8: Mouse brain whole tissue lysates

Lane 9: Mouse liver whole tissue lysates

Lane 10: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lane 11: Rat brain whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Mouse IgG(H+L), HRP Conjugated (Cat. No. LF101) at 1:5,000 dilution

Predicted band size: 85 kDa

Observed band size: 95 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-Hsp90 Mouse mAb [91M01L08]

Sample: HeLa cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibody: M011208 at 1:100 dilution and and α-tubulin Rabbit Monoclonal Antibody (Cat. No. LF213) at 1:100 dilution

Secondary antibody: Goat anti-Mouse (488) at 1:1,000 dilution (shown in green) and Goat anti-Rabbit (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).