Anti-ATP citrate lyase Mouse mAb [97K18M95]

抗体系列

Anti-ATP citrate lyase Mouse mAb [97K18M95]

Purified Recombinant Mouse Monoclonal Antibody

一键复制产品信息

货号	规格	价格
M010748
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

立即购买

Immunofluorescence - Anti-ATP citrate lyase Mouse mAb [97K18M95]

Sample: HeLa cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibody: M010748 at 1:100 dilution and and α-tubulin Rabbit Monoclonal Antibody (Cat. No. LF213) at 1:100 dilution

Secondary antibody: Goat anti-Mouse (488) at 1:1,000 dilution (shown in green) and Goat anti-Rabbit (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Western Blot - Anti-ATP citrate lyase Mouse mAb [97K18M95]

All lanes: M010748 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: MCF-7 (human breast adenocarcinoma epithelial cell) whole cell lysates

Lane 5: 293T (Human embryonic kidney cell) whole cell lysates

Lane 6: U87 (Human malignant glioblastoma epithelial cells) whole cell lysates

Lane 7: C2C12 (Mouse myoblasts epithelial cell) whole cell lysates

Lane 8: Mouse brain whole tissue lysates

Lane 9: Mouse liver whole tissue lysates

Lane 10: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lane 11: Rat brain whole tissue lysates

Lane 12: Rat liver whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Mouse IgG(H+L), HRP Conjugated (Cat. No. LF101) at 1:5,000 dilution

Predicted band size: 121 kDa

Observed band size: 121 kDa

Developed using the ECL technique (Cat. No. SQ201).

产品信息
说明书
已发表文献

Product Name	Anti-ATP citrate lyase Mouse mAb
Description	Purified Recombinant Mouse Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF (Cell)/ICC, ELISA
Dilution	WB 1:1,000~1:2,000; IF 1:100~1:200
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Clone No.	97K18M95
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant protein of human ATP citrate lyase
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	ACL; ATP citrate (pro-S) lyase; ATP citrate synthase; Citrate cleavage enzyme; EC 2.3.3.8; A730098H14RIK; ACLY; ATP CITRATE LYASE; ATPCL; AW538652; Cce; CITRATE LYASE; CLATP; MGC124629.
Molecular weight	Calculated MW: 121 kDa; Observed MW: 121 kDa
Uniprot ID	P53396
Gene ID	47
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-ATP citrate lyase Mouse mAb [97K18M95] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]

Background

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]

Cellular Location	Cytoplasm Cytosol

说明书下载

M010748 Anti-ATP citrate lyase Mouse mAb [97K18M95]

总篇数：加载中...

总影响因子：加载中...

最高影响因子：加载中...

加载中...

没有更多数据

产品中心

联系雅酶

上海市闵行区新骏环路760号1号楼3层

info@epizyme.cn

400-058-8030

技术支持(电话/微信)：

张老师 18217238522

罗老师 15021027255

李老师 18301721288

如有任何关于雅酶产品问题
欢迎来信咨询

雅酶微信公众号

抗体

蛋白提取与定量

蛋白电泳

蛋白免疫印迹

蛋白表达纯化与分离

蛋白质

ELISA试剂盒

核酸相关实验

微生物培养

设备与耗材

细胞培养

网站首页