Western Blot - Anti-TORC1 Mouse mAb [58L74L97]

All lanes: M010569 at 1:1,000 dilution

Lane 1: Mouse brain whole tissue lysates

Lane 2: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lane 3: Rat brain whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Mouse IgG(H+L), HRP Conjugated (Cat. No. LF101) at 1:5,000 dilution

Predicted band size: 67 kDa

Observed band size: 78 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-TORC1 Mouse mAb [58L74L97]

Sample: HeLa cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibody: M010569 at 1:100 dilution and and α-tubulin Rabbit Monoclonal Antibody (Cat. No. LF213) at 1:100 dilution

Secondary antibody: Goat anti-Mouse (488) at 1:1,000 dilution (shown in green) and Goat anti-Rabbit (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).