Anti-Pyruvate Dehydrogenase E2 Mouse mAb [77L67L58]

抗体系列

Anti-Pyruvate Dehydrogenase E2 Mouse mAb [77L67L58]

Purified Recombinant Mouse Monoclonal Antibody

一键复制产品信息

货号	规格	价格
M010550
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

立即购买

Immunofluorescence - Anti-Pyruvate Dehydrogenase E2 Mouse mAb [77L67L58]

Sample: HeLa cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibody: M010550 at 1:100 dilution and and α-tubulin Rabbit Monoclonal Antibody (Cat. No. LF213) at 1:100 dilution

Secondary antibody: Goat anti-Mouse (488) at 1:1,000 dilution (shown in green) and Goat anti-Rabbit (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Western Blot - Anti-Pyruvate Dehydrogenase E2 Mouse mAb [77L67L58]

All lanes: M010550 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: MCF-7 (human breast adenocarcinoma epithelial cell) whole cell lysates

Lane 5: 293T (Human embryonic kidney cell) whole cell lysates

Lane 6: U87 (Human malignant glioblastoma epithelial cells) whole cell lysates

Lane 7: C2C12 (Mouse myoblasts epithelial cell) whole cell lysates

Lane 8: Mouse brain whole tissue lysates

Lane 9: Mouse heart whole tissue lysates

Lane 10: Mouse liver whole tissue lysates

Lane 11: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lane 12: Rat brain whole tissue lysates

Lane 13: Rat heart whole tissue lysates

Lane 14: Rat liver whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Mouse IgG(H+L), HRP Conjugated (Cat. No. LF101) at 1:5,000 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa

Developed using the ECL technique (Cat. No. SQ201).

产品信息
说明书
已发表文献

Product Name	Anti-Pyruvate Dehydrogenase E2 Mouse mAb
Description	Purified Recombinant Mouse Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF (Cell)/ICC, ELISA
Dilution	WB 1:1,000~1:2,000; IF 1:100~1:200
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Clone No.	77L67L58
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant protein of human Pyruvate Dehydrogenase E2
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	PDC-E2; PDCE2; DLTA; E2; PBC; 6332404G05Rik; noa; nrb; wu:fc14f10; wu:fc21f08; ODP2_BOVIN; DLAT; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; Pyruvate dehydrogenase complex component E2 (PDC-E2 \| PDCE2); 2.3.1.12; ODP2_HUMAN; 70 kDa mitochondrial autoantigen of primary biliary cirrhosis (PBC); M2 antigen complex 70 kDa subunit; ODP2_MOUSE; ODP2_RAT.
Molecular weight	Calculated MW: 69 kDa; Observed MW: 69 kDa
Uniprot ID	P10515
Gene ID	1737
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Pyruvate Dehydrogenase E2 Mouse mAb [77L67L58] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.[provided by RefSeq, Oct 2009]

Background

This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.[provided by RefSeq, Oct 2009]

Cellular Location	Mitochondrion matrix

说明书下载

M010550 Anti-Pyruvate Dehydrogenase E2 Mouse mAb [77L67L58]

总篇数：加载中...

总影响因子：加载中...

最高影响因子：加载中...

加载中...

没有更多数据

产品中心

联系雅酶

上海市闵行区新骏环路760号1号楼3层

info@epizyme.cn

400-058-8030

技术支持(电话/微信)：

张老师 18217238522

罗老师 15021027255

李老师 18301721288

如有任何关于雅酶产品问题
欢迎来信咨询

雅酶微信公众号

抗体

蛋白提取与定量

蛋白电泳

蛋白免疫印迹

蛋白表达纯化与分离

蛋白质

ELISA试剂盒

核酸相关实验

微生物培养

设备与耗材

细胞培养

网站首页