| 货号 | 规格 | 价格 |
|---|---|---|
| FL135 | ||
| 10μg | ¥838.00 | |
| 100μg | ¥3580.00 | |
| 1mg | ¥17600.00 |
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| Appearance | Sterile filtered White lyophilized (freeze-dried) powder. |
|---|---|
| Purity | > 96% by SDS-PAGE or HPLC. |
| Endotoxin | < 0.01 EU/μg of rHuIL-18 protein as determined by LAL method. |
| Expression System | Expressed in E. coli. |
| Species | Human |
| Tag | Tag free. |
| Activity | Fully biologically active when compared to standard substance. Measured by its ability to induce IFN-gamma secretion by KG-1 human acute myelogenous leukemia cells. The ED50 for this effect is 1.5-15 ng/ml. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution | Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Accession # | Q14116 Tyr37-Asp193 |
|---|---|
| Amino acid sequence | YFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED |
| Molecular weight | Approximately 18.2 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids. |
| Synonyms | Iboctadekin, IFN-gamma-inducing factor, IL-1F4, Interleukin-1 gamma |
| Stability & Storage | Shipped on wet ice. For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution. |
| Precautions | Recombinant Human IL-18 is for research use only and not for use in diagnostic or therapeutic procedures. |
| Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation. IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 Rβ to form the signaling receptor complex. Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs. IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst. In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis. Alternative splicing in human ovarian cancer generates an isoform that is resistant to Caspase-1 activation. A cell surface form can be expressed on M-CSF induced macrophages and released in response to bacterial endotoxin. Mature human IL-18 shares approximately 63% amino acid sequence identity with mouse and rat IL-18. Recombinant IL-18 is a single non-glycosylated polypeptide chain containing 157 amino acids |
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