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Recombinant Porcine IFN-alpha1b

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货号 规格 价格
FL127
5μg ¥198.00
20μg ¥408.00
50μg ¥838.00
100μg ¥1380.00
1mg ¥5480.00

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Recombinant Porcine IFN-alpha1b

Product Specifications

Appearance Sterile filtered White lyophilized (freeze-dried) powder.
Purity > 96% by SDS-PAGE or HPLC.
Endotoxin < 0.1 EU/μg of rPoIFN-α1b protein as determined by LAL method.
Expression System Expressed in E. coli.
Species Porcine
Tag Tag free.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCL, 300 mM NaCl, 0.02% (v/v) Tween-20, pH 8.5.
Reconstitution Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.
Accession # P49879 Cys24-Glu189
Amino acid sequence CDLPQTHSLAHTRALRLLAQMRRISPFSCLDHRRDFGSPHEAFGGNQVQKAQAMALVHEMLQQTFQLFSTEGSAAAWNESLLHQFYTGLDQQLRDLEACVMQEAGLEGTPLLEEDSIRAVRKYFHRLTLYLQEKSYSPCAWEIVRAEVMRSFSSSRNLQDRLRKKE
Molecular weight Approximately 19.15 kDa, a single non-glycosylated polypeptide chain containing 166 amino acids.
Synonyms Interferon alpha‐2, Interferon alpha A, Type I interferon
Stability & Storage Shipped on wet ice. For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 12 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.
Precautions Recombinant Porcine IFN-alpha1b is for research use only and not for use in diagnostic or therapeutic procedures.

Background

IFN-αs are proteins secreted by leukocyte. They are mainly involved in innate immune response against viral infection. The IFN-α family has 13 subtypes and 23 different variants. The individual proteins have molecular masses between 19-26kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. The recombinant porcine IFN-α1 is a 19.15kDa protein consisting of 166 amino acid residues.

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