| 货号 | 规格 | 价格 |
|---|---|---|
| FL122 | ||
| 1mg | ¥2980.00 | |
| 10mg | ¥15300.00 | |
| 100mg | ¥76500.00 | |
| 1g | ¥297500.00 |
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| Appearance | Sterile filtered White lyophilized (freeze-dried) powder. |
|---|---|
| Purity | > 96% by SDS-PAGE or HPLC, dimers and high molecular weight impurities: <4% by SEC-HPLC. |
| Endotoxin | < 0.01 EU/μg of rCRM197 protein as determined by LAL method. |
| Expression System | Expressed in E. coli. |
| Tag | Tag free. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 0.025% (v/v) Tween-80 pH7.4. |
| Reconstitution | Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Accession # | AMV91693 Gly2-Ser536 |
|---|---|
| Amino acid sequence | GADDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDDDWKEFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELGLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEINFETRGKRGQDAMYEYMAQACAGNRVRRSVGSSLSCINLDWDVIRDKTKTKIESLKEHGPIKNKMSESPNKTVSEEKAKQYLEEFHQTALEHPELSELKTVTGTNPVFAGANYAAWAVNVAQVIDSETADNLEKTTAALSILPGIGSVMGIADGAVHHNTEEIVAQSIALSSLMVAQAIPLVGELVDIGFAAYNFVESIINLFQVVHNSYNRPAYSPGHKTQPFLHDGYAVSWNTVEDSIIRTGFQGESGHDIKITAENTPLPIAGVLLPTIPGKLDVNKSKTHISVNGRKIRMRCRAIDGDVTFCRPKSPVYVGNGVHANLHVAFHRSSSEKIHSNEISSDSIGVLGYQKTVDHTKVNSKLSLFFEIKS |
| Molecular weight | Approximately 58.4 kDa, a single non-glycosylated polypeptide chain containing 535 amino acids |
| Stability & Storage | Shipped on wet ice. For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 12 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution. |
| Precautions | Recombinant Diphtheria Toxin Mutant CRM197 is for research use only and not for use in diagnostic or therapeutic procedures. |
| CRM197 is a non-toxic mutant of diphtheria toxin having a single amino acid substitution of glycine with glutamic acid. CRM197 is a well-defined protein and functions as a carrier for polysaccharides and haptens making them more immunogenic. CRM197 is utilized as a carrier protein in a number of approved conjugate vaccines for diseases caused by Streptococcus pneumoniae, Haemophilus influenzae b and Neisseria meningitidis. Recombinant CRM197 is a 58.4kDa globular protein containing 535 amino acids. |
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