| 货号 | 规格 | 价格 |
|---|---|---|
| FL116 | ||
| 2μg | ¥328.00 | |
| 10μg | ¥998.00 | |
| 50μg | ¥2580.00 | |
| 100μg | ¥4980.00 | |
| 250μg | ¥8280.00 | |
| 500μg | ¥12500.00 | |
| 1mg | ¥23800.00 |
立即购买
| Appearance | Sterile filtered White lyophilized (freeze-dried) powder. |
|---|---|
| Purity | > 97% by SDS-PAGE or HPLC. |
| Endotoxin | < 0.1 EU/μg of rHuGDNF protein as determined by LAL method. |
| Expression System | Expressed in E. coli. |
| Species | Human |
| Tag | Tag free. |
| Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.1 ng/ml, corresponding to a specific activity of ≥ 5.0×10^7 IU/mg. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 500 mM NaCl, 0.05% (v/v) Tween-20, pH 7.4. |
| Reconstitution | Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Accession # | P39905 Ser77-Ile211 |
|---|---|
| Amino acid sequence | SPDKQMAVLPRRERNRQAAAANPENSRGKGRRGQRGKNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCDAAETTYDKILKNLSRNRRLVSDKVGQACCRPIAFDDDLSFLDDNLVYHILRKHSAKRCGCI |
| Molecular weight | Approximately 30.1 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains. |
| Synonyms | ATF, ATF-1 |
| Stability & Storage | Shipped on wet ice. For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution. |
| Precautions | Recombinant Human GDNF is for research use only and not for use in diagnostic or therapeutic procedures. |
| GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons. Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional human GDNF ligand is a disulfide-linked homodimer consisting of two 15kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinacocytes and skeletal muscle cells. Rat and human mature GDNF shows 93% sequence identity and have species cross-reactivity. The calculated molecular weight of Recombinant Human GDNF is 30.1kDa. |
总篇数：加载中...
总影响因子：加载中...
最高影响因子：加载中...
上海市闵行区新骏环路760号1号楼3层
400-058-8030
技术支持(电话/微信)：
张老师 18217238522
罗老师 15021027255
李老师 18301721288
如有任何关于雅酶产品问题
欢迎来信咨询
雅酶微信公众号
Copyright © 2023上海雅酶生物医药科技有限公司（Epizyme Biotech）
沪ICP备2021002422号
