| 货号 | 规格 | 价格 |
|---|---|---|
| FL108 | ||
| 1mg | ¥198.00 | |
| 5mg | ¥558.00 | |
| 50mg | ¥2680.00 | |
| 1g | ¥10200.00 |
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| Appearance | Sterile filtered White lyophilized (freeze-dried) powder. |
|---|---|
| Purity | > 95% by SDS-PAGE or HPLC. |
| Endotoxin | < 0.1 EU/μg of Cys-Protein G protein as determined by LAL method. |
| Expression System | Expressed in E. coli. |
| Tag | Tag free. |
| Concentration for 1 unity DO at 280 nm | 0.729mg/ml |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PB, pH 7.4. |
| Reconstitution | Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Accession # | P19909 Leu298-Glu495 with an N-terminal Cys |
|---|---|
| Amino acid sequence | CLPKTDTYKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTLKGETTTKAVDAETAEKAFKQYANDNGVDGVWTYDDATKTFTVTE |
| Molecular weight | Approximately 21.9 kDa, and it migrates with an apparent molecular mass of 40 kDa in SDS-PAGE. |
| Stability & Storage | Shipped on wet ice. For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution. |
| Precautions | Recombinant Cys-Protein G is for research use only and not for use in diagnostic or therapeutic procedures. |
| Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcic. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F (ab') fragments of IgG. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology Recombinant Cys-Protein G is a single non-glycosylated polypeptide chain containing 201 amino acids. and there is an N-terminal cysteine for Site-Specific Conjugation. |
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