| 货号 | 规格 | 价格 |
|---|---|---|
| FL074 | ||
| 5μg | ¥198.00 | |
| 20μg | ¥668.00 | |
| 100μg | ¥2980.00 | |
| 500μg | ¥7680.00 | |
| 1mg | ¥15300.00 |
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| Appearance | Sterile filtered White lyophilized (freeze-dried) powder. |
|---|---|
| Purity | > 97% by SDS-PAGE or HPLC. |
| Endotoxin | < 0.01 EU/μg of rHuCNTF protein as determined by LAL method. |
| Expression System | Expressed in E. coli. |
| Species | Human |
| Tag | Tag free. |
| Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 150 ng/ml, corresponding to a specific activity of ≥ 6.7×10^3 IU/mg. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, 3% (v/v) Trehalose, 0.02% (v/v) Tween-20, pH 7.0. |
| Reconstitution | Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Accession # | P26441 Ala2-Met200 |
|---|---|
| Amino acid sequence | AFTEHSPLTPHRRDLCSRSIWLARKIRSDLTALTQSYVKHQGLNKNINLDSADGMPVASTDQWSQLTQAQRLQQNLQAYRTFHVLLARLLQDQQVHFTPTQGDFHQAIHTLLLQVAAFAYQIQQLMILLQYKIPRNQADGMPINVGDGGLFQKKLWGLKVLQQLSQWTVRSIHDLRFISSHQTGIPARGSHYIANNKKM |
| Molecular weight | Approximately 22.9 kDa, a single non-glycosylated polypeptide chain containing 199 amino acids. |
| Synonyms | Ciliary neurotrophic factor, CNTF, HCNTF |
| Stability & Storage | Shipped on wet ice. For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution. |
| Precautions | Recombinant Human CNTF is for research use only and not for use in diagnostic or therapeutic procedures. |
| Ciliary neurotrophic factor (CNTF) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. It was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. The protein is also a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is highly conserved across species and exhibits cross-species bioactivity. Recombinant Human CNTF is a 22.9kDa globular protein containing 199 amino acid residues. |
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