| 货号 | 规格 | 价格 |
|---|---|---|
| FL061 | ||
| 2μg | ¥198.00 | |
| 10μg | ¥998.00 | |
| 50μg | ¥2780.00 | |
| 100μg | ¥4980.00 | |
| 500μg | ¥11900.00 | |
| 1mg | ¥23800.00 |
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| Appearance | Sterile filtered White lyophilized (freeze-dried) powder. |
|---|---|
| Purity | > 98% by SDS-PAGE or HPLC. |
| Endotoxin | < 0.01 EU/μg of rMuIL-33 protein as determined by LAL method. |
| Expression System | Expressed in E. coli. |
| Species | Mouse |
| Tag | Tag free. |
| Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using mouse D10S cells is less than 0.5 ng/ml, corresponding to a specific activity of ≥ 2.0×10^6 IU/mg. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution | Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Accession # | Q8BVZ5 Ser109-Ile266 |
|---|---|
| Amino acid sequence | SIQGTSLLTQSPASLSTYNDQSVSFVLENGCYVINVDDSGKDQEQDQVLLRYYESPCPASQSGDGVDGKKLMVNMSPIKDTDIWLHANDKDYSVELQRGDVSPPEQAFFVLHKKSSDFVSFECKNLPGTYIGVKDNQLALVEEKDESCNNIMFKLSKI |
| Molecular weight | Approximately 17.5 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids. |
| Synonyms | Interleukin-33, IL-1 F11, NF-HEV |
| Stability & Storage | Shipped on wet ice. For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution. |
| Precautions | Recombinant Mouse IL-33 is for research use only and not for use in diagnostic or therapeutic procedures. |
| IL-33 is a number of IL-1 superfamily, secreted by high endothelial venules at high levels, which is found in tonsils, peyer patches and mesenteric lymph nodes, but not in placenta. It binds and signals through the IL-1RL1/ST2 receptor, to activate NF-κB and MAP kinases. IL-33 induces production of TH2 cell related cytokines, including IL-4, IL-5 and IL-13, and exerts multiple inflammation related bioactivities. Full length and mature mouse IL-33 share approximately 55% and 90% aa sequence identity with human and rat IL-33, respectively. Recombinant Mouse IL-33 is a 17.5kDa protein containing 158 amino acid. |
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