| 货号 | 规格 | 价格 |
|---|---|---|
| FL055 | ||
| 5μg | ¥198.00 | |
| 20μg | ¥748.00 | |
| 50μg | ¥1780.00 | |
| 100μg | ¥3080.00 | |
| 1mg | ¥15300.00 |
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| Appearance | Sterile filtered White lyophilized (freeze-dried) powder. |
|---|---|
| Purity | > 96% by SDS-PAGE or HPLC. |
| Endotoxin | < 0.1 EU/μg of rHu4-1BBL protein as determined by LAL method. |
| Expression System | Expressed in E. coli. |
| Species | Human |
| Tag | Tag free. |
| Activity | Fully biologically active when compared to standard. The ED50 as determined by stimulation of IL-8 production using human PBMC is less than 10 ng/ml, corresponding to a specific activity of ≥ 1.0×10^5 IU/mg. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution | Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Accession # | P41273 Arg71-Glu254 |
|---|---|
| Amino acid sequence | REGPELSPDDPAGLLDLRQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVTPEIPAGLPSPRSE |
| Molecular weight | Approximately 19.4 kDa, a single non-glycosylated polypeptide chain containing 184 amino acids. |
| Synonyms | CD137L |
| Stability & Storage | Shipped on wet ice. For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution. |
| Precautions | Recombinant Human 4-1BB Ligand/TNFSF9 is for research use only and not for use in diagnostic or therapeutic procedures. |
| 4-1BBL is a member of the tumor necrosis factor (TNF) receptor family. This receptor contributes to the clonal expansion, survival, and development of T cells. In addition, 4-1BBL expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. The human 4-1BBL gene codes for a 254 amino acid type II transmembrane containing a 28 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 205 amino acid extracellular domain (ECD) . The human 41BBL ECD shares 32% and 35% a.a. identity with murine and rat ECD. Recombinant Human 4-1BB Ligand/TNFSF9 is a 19.4kDa non-glycosylated protein containing 184 amino acid residues. |
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