| 货号 | 规格 | 价格 |
|---|---|---|
| FL042 | ||
| 5μg | ¥198.00 | |
| 20μg | ¥668.00 | |
| 50μg | ¥1480.00 | |
| 100μg | ¥2980.00 | |
| 1mg | ¥15300.00 |
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| Appearance | Sterile filtered White lyophilized (freeze-dried) powder. |
|---|---|
| Purity | > 98% by SDS-PAGE or HPLC. |
| Endotoxin | < 0.01 EU/μg of rHuBetacellulin protein as determined by LAL method. |
| Expression System | Expressed in E. coli. |
| Species | Human |
| Tag | Tag free. |
| Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of ≥ 2.0×10^7 IU/mg.. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, pH 7.2. |
| Reconstitution | Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Accession # | P35070 Asp32-Tyr111 |
|---|---|
| Amino acid sequence | DGNSTRSPETNGLLCGDPEENCAATTTQSKRKGHFSRCPKQYKHYCIKGRCRFVVAEQTPSCVCDEGYIGARCERVDLFY |
| Molecular weight | Approximately 9.0 kDa, a single non-glycosylated polypeptide chain containing 80 amino acids. |
| Synonyms | BTC |
| Stability & Storage | Shipped on wet ice. For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution. |
| Precautions | Recombinant Human Betacellulin is for research use only and not for use in diagnostic or therapeutic procedures. |
| Betacellulin (BTC) is a member of the EGF family of cytokines that also includes EGF, TGF-a, Amphiregulin, HB-EGF, Epiregulin, Tomoregulin and the Neuregulin. It is expressed in most tissues including kidney, uterus, liver and pancreas. BTC also presents in body fluids, including serum, milk, and colostrum. At the amino acid sequence level, human mature BTC protein exhibits 80% identity with mouse BTC protein. The protein binds to EGFR, ERBB4 and other EGF receptor family members and acts as a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Recombinant Human Betacellulin is a 9.0kDa globular protein containing 80 amino acid residues，which comprises the mature EGF homologous portion of the Betacellulin protein. |
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