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Anti-Smad1 Rabbit mAb [28D91L21]

Purified Rabbit Recombinant Monoclonal Antibody

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名称: Anti-Smad1 Rabbit mAb [28D91L21] 货号: R013764 应用: WB, IHC-P, IF (Cell), ELISA 稀释比: WB 1:8,000; IHC-P 1:200; IF 1:100 种属反应性: Human, Mouse 规格: 100µL; 50µL; 20µL 价格: ¥1780.00; ¥1080.00; ¥588.00 链接: http://www.epizyme.cn/pro_show_kt.aspx?productid=24938
货号 规格 价格
R013764
100µL ¥1780.00
50µL ¥1080.00
20µL ¥588.00

Immunofluorescence - Anti-Smad1 Rabbit mAb [28D91L21]

Sample: Hela cells

The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R013764 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Immunofluorescence - Anti-Smad1 Rabbit mAb [28D91L21]

Sample: Hela cells

The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibody: R013764 at 1:100 dilution

Secondary antibody: Goat anti-Rabbit (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Immunofluorescence - Anti-Smad1 Rabbit mAb [28D91L21]

Sample: A431 cells

The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R013764 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Western Blot - Anti-Smad1 Rabbit mAb [28D91L21]

All lanes: R013764 at 1:8,000 dilution

Lane 1: A431 (human epidermoid carcinoma cell line) whole cell lysates

Lane 2: Jurkat (human T lymphocytic leukemia cell) whole cell lysates

Lane 3: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: SW620 (human colorectal carcinoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

Developed using the ECL technique (Cat. No. SQ201).

Western Blot - Anti-Smad1 Rabbit mAb [28D91L21]

All lanes: R013764 at 1:1,000 dilution

Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysates

Lane 3: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: T24 (human bladder cancer epithelial cell) whole cell lysates

Lane 5: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysates

Lane 6: U2OS (human osteosarcoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-Smad1 Rabbit mAb [28D91L21]

Sample: Paraformaldehyde-fixed, paraffin embedded human breast carcinoma tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013764 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-Smad1 Rabbit mAb [28D91L21]

Sample: Paraformaldehyde-fixed, paraffin embedded mouse brain tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013764 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-Smad1 Rabbit mAb [28D91L21]

Sample: Paraformaldehyde-fixed, paraffin embedded human endometrial cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013764 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Product Name Anti-Smad1 Rabbit mAb
Description Purified Rabbit Recombinant Monoclonal Antibody
Application
  • Applications Legend:
  • WB=Western Blotting
  • IHC-P=Immunohistochemistry (Paraffin)
  • IHC-F=Immunohistochemistry (Frozen)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF (Cell), ELISA
Dilution WB 1:8,000; IHC-P 1:200; IF 1:100
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone No. 28D91L21
Isotype IgG
Target / Specificity A synthesized peptide derived from human Smad1
Format Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.
Gene ID 4086
Synonyms Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, JV4-1, Mad-related protein 1, SMAD family member 1, SMAD 1, Smad1, hSMAD1, Transforming growth factor-beta-signaling protein 1, BSP-1, SMAD1, BSP1, MADH1, MADR1.
Calculated MW 52 kDa
Primary Accession Q15797
Storage Shipped at 4℃. Upon delivery aliquot. Store at 4℃ short term (1~2 weeks). Store at -20℃ for 2 years. Avoid freeze / thaw cycles.
Precautions Anti-Smad1 Rabbit mAb [28D91L21] is for research use only and not for use in diagnostic or therapeutic procedures.
Background Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.
Cellular Location Cytoplasm. Nucleus Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 (PubMed:15647271). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15647271). Exported from the nucleus to the cytoplasm when dephosphorylated (By similarity) {ECO:0000250|UniProtKB:P70340, ECO:0000269|PubMed:15647271}.
Tissue Location Ubiquitous. Highest expression seen in the heart and skeletal muscle.

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