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Anti-RAB7A Mouse mAb [24D164D1]

Purified Recombinant Mouse Monoclonal Antibody

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名称: Anti-RAB7A Mouse mAb [24D164D1] 货号: M900003 应用: WB, IF 稀释比: WB 1:1,000; IF:1:100 种属反应性: Human 规格: 100µL; 50µL; 20µL 价格: ¥1780.00; ¥1080.00; ¥588.00 链接: http://www.epizyme.cn/pro_show_kt.aspx?productid=24869
货号 规格 价格
M900003
100µL ¥1780.00
50µL ¥1080.00
20µL ¥588.00

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.01% Saponin permeabilized A-431 cells labeling anti-RAB7A at 1:100 dilution, followed by Goat Anti-mouse IgG H+L secondary antibody at 1:1,000 dilution (green). The nuclear counter stain is DAPI (blue).

Western Blot analysis of RAB7A expression in cell lines of HEK293T, Hela, Hep-G2, and A-431. Lysates at 20 μg per lane. Anti-RAB7A Mouse mAb was used as the primary antibody (1:1,000) and goat anti-mouse IgG-HRP (LF101) was used as the secondary antibody (1:5,000). Chemiluminescent detection was performed using Omni-ECL™ Femto Light Chemiluminescence Kit (SQ201).

Product Name Anti-RAB7A Mouse mAb
Description Purified Recombinant Mouse Monoclonal Antibody
Application
  • Applications Legend:
  • WB=Western Blotting
  • IHC-P=Immunohistochemistry (Paraffin)
  • IHC-F=Immunohistochemistry (Frozen)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF
Dilution WB 1:1,000; IF:1:100
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone No. 24D164D1
Isotype IgG
Target / Specificity This RAB7A antibody is generated from mice immunized with a SHC (Shrimp Hemocyanin) conjugated synthetic peptide between 187-201 amino acids from human RAB7A.
Format Purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3. This antibody is purified through a protein G column.
Gene ID 7879
Synonyms RAB7A_HUMAN; Ras-related protein Rab-7a; EC:3.6.5.2; RAB7
Calculated MW 23 kDa
Primary Accession P51149
Other Accession NP_004628.4
Antigen Region 187-201 aa
Storage Shipped at 4℃. Upon delivery aliquot. Store at 4℃ short term (1~2 weeks). Store at -20℃ for 2 years. Avoid freeze / thaw cycles.
Precautions Anti-RAB7A Mouse mAb is for research use only and not for use in diagnostic or therapeutic procedures.
Background Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins playing a key role in the regulation of endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA (PubMed:11179213, PubMed:12944476, PubMed:14617358, PubMed:20028791, PubMed:21255211).
Cellular Location Cytoplasmic vesicle; Endosome; Lipid droplet; Lysosome; Membrane; Mitochondrion.

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