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Anti-ZO-1 Mouse mAb [24D02C18]

Purified Mouse Monoclonal Antibody

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名称: Anti-ZO-1 Mouse mAb [24D02C18] 货号: M900002 应用: WB, IF 稀释比: WB 1:1,000~1:5,000; IF:1:500~1:1,000 种属反应性: Canis, Rat, Human, Mouse 规格: 100µL; 50µL; 20µL 价格: ¥1780.00; ¥1080.00; ¥588.00 链接: http://www.epizyme.cn/pro_show_kt.aspx?productid=24868
货号 规格 价格
M900002
100µL ¥1780.00
50µL ¥1080.00
20µL ¥588.00

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.01% Saponin permeabilized CACO-2 cells labeling Anti-ZO-1 Mouse mAb at 1:500 dilution, followed by Goat Anti-Rabbit IgG H+L secondary antibody at 1:1,000 dilution (green). The nuclear counter stain is DAPI (blue).

Western-blot analysis of ZO-1 expression in cell lines of Hela, Hep-G2, CACO-2 and HEK293T . Lysates at 20 μg per lane. Anti-ZO-1 was used as the primary antibody (1:1,000) and goat anti-mouse IgG-HRP (LF101) was used as the secondary antibody (1:5,000). Chemiluminescent detection was performed using Omni-ECL™ Femto Light Chemiluminescence Kit (SQ201).

Immunohistochemical analysis of human kidney tissue labeling Anti-ZO-1 Mouse mAb at 1:100 dilution followed by a ready-to-use Goat Anti-mouse IgG H+L (HRP). Counterstained with hematoxylin.

Product Name Anti-ZO-1 Mouse mAb
Description Purified Mouse Monoclonal Antibody
Application
  • Applications Legend:
  • WB=Western Blotting
  • IHC-P=Immunohistochemistry (Paraffin)
  • IHC-F=Immunohistochemistry (Frozen)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF
Dilution WB 1:1,000~1:5,000; IF:1:500~1:1,000
Reactivity Canis, Rat, Human, Mouse
Host Mouse
Clonality Monoclonal
Clone No. 24D02C18
Isotype IgG
Target / Specificity This ZO-1 antibody is generated from mice immunized with a SHC (Shrimp Hemocyanin) conjugated synthetic peptide between 39-53 amino acids from homo sapiens (Human).
Format Purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3. This antibody is purified through a protein G column.
Gene ID 7082
Synonyms Tight junction protein 1; Tight junction protein ZO-1; Tight junction protein ZO1
Calculated MW 230 kDa
Primary Accession Q07157
Antigen Region 39-53 aa
Storage Shipped at 4℃. Upon delivery aliquot. Store at 4℃ short term (1~2 weeks). Store at -20℃ for 2 years. Avoid freeze / thaw cycles.
Precautions Anti-ZO-1 Mouse mAb is for research use only and not for use in diagnostic or therapeutic procedures.
Background ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
Cellular Location Cell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
Tissue Location The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.

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